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Amplification efficiency of quantitative polymerase chain reaction (qPCR) assay using stool or buffer as matrix. Twenty-five samples prepared in stool (squares) and 25 samples prepared in buffer (triangles) were evaluated in triplicate by qPCR. Samples contained 1, 2, 10, 50, or 250 enumerated, purified Schistosoma japonicum eggs. Regression lines and equations are indicated for each sample matrix.
