Table 1.
Recovery of Schistosoma japonicum eggs spiked into uninfected buffalo stool by qPCR*
EPG† | No. samples prepared‡ | Measured EPG by qPCR | |
---|---|---|---|
Mean ± SD | Range | ||
1 | 3 | 0.9 ± 0.4 | 0.8–1.3 |
2 | 3 | 2.5 ± 1.0 | 1.6–3.8 |
5 | 5 | 7.5 ± 1.2 | 6.2–8.7 |
10 | 5 | 10.0 ± 2.1 | 7.4−13.0 |
20 | 5 | 20.8 ± 4.6 | 14.2–26.9 |
40 | 5 | 34.5 ± 3.8 | 29.0–36.4 |
80 | 5 | 82.2 ± 10.1 | 70.8–98.2 |
qPCR = quantitative polymerase chain reaction; EPG = eggs per gram (of feces).
EPG of purified enumerated S. japonicum eggs spiked into uninfected buffalo stool.
Each sample was tested in triplicate.