Table 1.

Primers used for the reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) assay, sequencing of the LAMP product;^* are qRT-PCR primers taken from Van der Meide (2008)¹⁴

Primer name	Purpose	Sequence 5' to 3'
FIP	LAMP assay	CCA CGT CTT GTG GTG CCA TTC C-GG GGA GTA CGT TCG CAA G
BIP	LAMP assay	CGG GGA ACT TTA CCA GAT CCG G-AC CAC CAT TCA GGG AAT CGA
F3	LAMP assay	CGC GAA AGC TTT GAG GTT AC
B3	LAMP assay	ACA AAT CAC TCC ACC GAC C
LF	LAMP assay	GTC AAT TTC TTT AAG TTT CAC T
LB	LAMP assay	TGA GGA TTG ACA GAT GGA GTG TTC
F2	Sequencing LAMP product	GGG GAG TAC GTT CGC AAG
B2	Sequencing LAMP product	ACCACCATTCAGGGAATCGA
qRT-PCR forward primer*	qRT-PCR	CCA AAG TGT GGA GAT CGA AG
qRT-PCR reverse primer*	qRT-PCR	GGC CGG TAA AGG CCG AAT AG
qRT-PCR probe*	qRT-PCR	6FAM ACC ATT GTA GTC CAC ACT GC NFQ MGB

^*The 5'-fluorescently labeled probe [6-carboxyfluorescein (FAM)] were conjugated to a non-fluorescent quencher (NFQ) and a minor groove binder probe group (MGB) at the 3' end.

^“-“Denotes the space between F1c & F2 and B1c & B2.