Figure 1.

Resveratrol competes for estrogen receptor binding. (A) Structures of resveratrol, DES, and estradiol. (B) Estrogen receptor binding assays were performed as described in Experimental Procedures by using 0.1 nM (circles), 0.3 nM (triangles), or 1.0 nM (squares). ¹²⁵I-estradiol competed with the indicated concentrations of resveratrol (open symbols) or unlabeled estradiol (filled circles). Each point represents the mean and range of duplicate assays after subtraction of nonspecific binding. All results are shown as percentage of binding in the absence of competitor.