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. 1997 Dec 9;94(25):14138–14143. doi: 10.1073/pnas.94.25.14138

Figure 3.

Figure 3

Transcriptional activation by resveratrol is estrogen receptor-mediated. (A) Dependence on an ERE. MCF-7 cells were transfected with tk109-luc or ERE-tk109-luc, incubated with the indicated concentrations of resveratrol or estradiol, and assayed for luciferase activity. Results are shown as fold induction compared with control-treated cells. (B) Inhibition by estrogen antagonists. MCF-7 cells were transfected with ERE-tk109-luc, incubated with no agonist, estradiol (0.1 nM), or resveratrol (10 μM) or with the agonists plus the antagonists tamoxifen (1 μM) or ICI 182780 (100 nM). (C) Requirement for the estrogen receptor. MDA-MB-231 cells were transfected with 1 μg/well ERE2-tk109-luc and 10 ng/well of pSG5 or pSG5-HEGO, incubated with the indicated concentrations of resveratrol or estradiol, and assayed for luciferase activity. All values are means ± SEM of triplicate transfections.