Figure 5.

Effect of resveratrol on estrogen-regulated transcription and growth. (A) MCF-7 cells were treated with resveratrol or estradiol, and progesterone receptor (PR) mRNA was measured by RT-PCR. Results are normalized to glyceraldehyde-3-phosphate dehydrogenase mRNA and shown as fold induction relative to control (0.1% ethanol). Similar results were obtained in an independent experiment. (B) T47D cells were cultured in the presence of 0.1 nM estradiol or 10 μM resveratrol with or without 100 nM ICI 182780 or in control medium. Cell density was determined at the indicated intervals. Each point represents the mean ± SEM of eight replicate wells (n = 16 for day 0).