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. 1994 Feb;38(2):388–391. doi: 10.1128/aac.38.2.388

Expression of reverse transcriptase from feline immunodeficiency virus in Escherichia coli.

T W North 1, G L Hansen 1, Y Zhu 1, J A Griffin 1, C K Shih 1
PMCID: PMC284465  PMID: 7514859

Abstract

Reverse transcriptase from feline immunodeficiency virus (FIV) has been cloned and expressed in Escherichia coli. We have purified this recombinant enzyme and shown that it is a 66-kDa protein that is indistinguishable from virion-derived FIV reverse transcriptase in sensitivity to the 5'-triphosphates of 3'-azido-3'-deoxythymidine and the four 2',3'-dideoxynucleosides. The availability of large quantities of the FIV reverse transcriptase will allow more detailed physical and pharmacological studies.

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Selected References

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