Amplification of Shigella dysenteriae DNA using the single channel TFRT. PCR amplification (35 cycles) of ipaH gene from Shigella dysenteriae on a conventional Peltier-based thermocycler and thin-film resistive thermocycler (TFRT) in a single-capillary mode. (A) gel, (B) capillaries. M – 123 bp DNA ladder (see Note 10), 1 – negative control, 2 – TFRT (37 min), 3 – TFRT (20 min), 4 – TFRT (17.5 min), 5 – Conventional thermocycler (122 min).