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Assay performance in other matrixes. PbTx-3 (1.2 ng/mL, final concentration) was spiked into a 100% acetone extract of oysters, human urine, human serum, and a control (80% methanol) extract. Each extract was then analyzed in triplicate. The 1:2 dilutions of both serum and urine (shaded bars) indicated a small amount (22 and 10%, respectively) of matrix interference. All other samples were 97–105% of the control value.
