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. Author manuscript; available in PMC: 2011 Feb 15.
Published in final edited form as: J Immunol. 2010 Jan 8;184(4):1675–1679. doi: 10.4049/jimmunol.0903539

Figure 1. pLN γδ T cells preferentially express IL-17 following activation.

Figure 1

(A) LN cells were ex vivo stimulated with PMA/ionomycin and subsequently stained for CD4, NK1.1, CD8, B220, and IL-17. (B) LN cells stimulated as above were stained for B220, CD4, CD8, IL-17, and γδ TCR. Shown is γδ T cell IL-17 expression by non-B/non-T cells. (C) γ δ, CD4, and CD8 T cells from the indicated tissues were examined for the surface expression of CD44 and CD62L. (D) IL-17 expression of CD44low and CD44high γδ T cells (spleen and pLN) was examined by intracellular staining. (E) γδ T cells from the indicated tissues were stimulated and stained for IL-17 and CD27. (F) Proportions of IFNγ- and of IL-17-producing γδ T cells (and CD4 T cells) in the indicated tissues were examined. Shown is the mean ± SD (n=4). (G) Cells isolated from the indicated tissues were subsequently stimulated with PMA/Ionomycin, and cytokine production was determined by flow cytometric analysis. Shown are cytokine profiles of γδ TCR+ gated cells. All the experiments were repeated more than twice and similar results were observed. **, p<0.01, ***, p<0.001.