Table 2.
Capacity of specific cucurbit phloem sap proteins to interact with plasmodesmata to induce an increase in SEL and mediate their own cell-to-cell transport in cotyledons of C. maxima
| Injected material | Protein concentration,* μg⋅μl−1 | Microinjections† |
|---|---|---|
| LYCH | — | 15 (15; 100%) |
| 20-kDa FITC-dextran | — | 0 (15; 0%) |
| 17-kDa protein fraction‡ | 0.6 | 12 (17; 71%) |
| 24-kDa protein fraction | 1.0 | 14 (15; 93%) |
| 30-kDa protein fraction | 1.5 | 11 (14; 79%) |
| 40-kDa protein fraction | 1.4 | 5 (16; 31%) |
| 52-kDa protein fraction | 1.2 | 12 (15; 80%) |
Represents phloem sap protein concentration back-loaded into the microinjection pipette.
Details as in Table 1.
Each protein fraction was coinjected with 20-kDa FITC-dextran.