FIG. 2.

Effects of glucose and FFAs on SIRT1 gene and protein expression in THP-1 cells. A and B: THP-1 cells were cultured under normal (5.5 mmol/l) or high (20 mmol/l) glucose for 3, 24, and 48 h. SIRT1 gene (A) and protein (B) expression analysis was performed by Q-PCR and Western blot, respectively. C and D: THP-1 cells were incubated in presence or absence of FFAs (500 μmol/l palmitic acid or 500 μmol/l linoleic acid) for 3 and 24 h. SIRT1 gene (C) and protein (D) expression analysis was performed by Q-PCR and Western blot, respectively. Data are expressed as mean of six experiments ± SE, *P < 0.05.