Skip to main content
. 2010 Jan 12;59(4):894–906. doi: 10.2337/db09-1024

FIG. 1.

FIG. 1.

Generation and characterization of transgenic mice that express Cre-activatable Socs3. A: Transgenic construct to overexpress Socs3 in a Cre-activatable way. In cells in which Cre is absent, Socs3 is not expressed because of the upstream stop cassette, which consists of four copies of polyadenylation signal (pA). In cells in which Cre is expressed, the stop cassette is removed and Socs3 expression is driven by the CMV promoter. Nomenclature of mice used in this study is listed. B: Transgenic mice carrying the Tg.Socs3-OE allele did not show a difference in body weight (Tg.Socs3-OE/+) compared with wild-type (+/+) controls (controls n = 6–30, Tg.Socs3-OE n = 5–20). C: Transgenic Socs3 expression is Cre dependent. Tg.Socs3-OE mice were crossed with mice carrying Nestin-Cre. Semiquantitative real-time RT-PCR showing differential expression of transgenic Socs3 (Tg.Socs3) in the hypothalamus where Nestin-Cre is expressed, and in liver where Nestin-Cre is not expressed. Primers were specific to the Socs3 transgene. Hyp, hypothalamus; Liv, liver. D: Socs3 mRNA expression was analyzed in 0.5-week-old control and Nestin-Socs3-OE mutant mice (controls n = 12, mutants n = 6). E: Hypothalamic Socs3 mRNA expression was analyzed in 0.5- and 8-week-old wild-type C57BL6/J mice (n = 6–8). All mice were fed a chow diet. *P < 0.05 by Student t test.