FIG. 4.

mTOR-S6 signaling is impaired in POMC neurons of POMC-Socs3-OE mice. A: The 6-week-old control and POMC-Socs3-OE mice were injected with either saline or leptin (3 mg/kg), and perfused 45 min later. Double immunofluorescence analysis was carried out to examine phosphorylation of S6 (pS6) in POMC neurons using antibodies against Ser235/236-S6 (green) and ACTH (red). B: Quantification of POMC and pS6-positive cells showed that pS6 was specifically reduced in POMC neurons but not in other cells in the arcuate nucleus of mutant mice. C: Percentage of POMC neurons that is positive for pS6 after either saline or leptin treatment. n = 3–5 sections per mouse from 3–5 mice per group between bregma −1.22 and −1.58. Data represent mean ± SEM. *P < 0.05, **P < 0.01 for comparisons shown as determined by Student t test. (A high-quality digital representation of this figure is available in the online issue.)