TABLE 2.

Effects of CB1 antagonism on fatty acid oxidation and parameters related to lipoprotein metabolism in liver explants

	HSHF	HSHF+SR
Palmitic acid oxidation (nmol · h−1 · g protein−1)	23.0 ± 13	22.0 ± 2.4
ApoB secretion (μg · h−1 · g protein−1)	155 ± 17*	123 ± 8†
ApoA secretion (μg · h−1 · g protein−1)	141 ± 2	140 ± 3
HDL-CE uptake ([3H]-CE dpm · h−1 · μg protein−1)	430 ± 119*	1,075 ± 139†

Data are means ± SE. Thin liver slices (∼200 μm) were obtained from mice fed an HSHF diet and treated either with 10 mg · kg⁻¹ · day⁻¹ of SR141716 (HSHF+SR; n = 5) or vehicle (HSHF; n = 5). For fatty acid oxidation and apo secretion, slices were incubated at 37°C in oxygenated William's medium E supplemented with l-carnitine (0.5 mmol/l) in the presence of 0.2 mmol/l of [1-¹⁴C] palmitic acid (55.5 GBq/mol) complexed to albumin (fatty acid/BSA molar ratio 2.5/1). After 4 h of incubation, slices were rinsed with cold PBS and immediately submitted to lipid extraction for counting of labelled CO₂ and acid-soluble products, while the incubation medium was used for determination of apoB and apoA secreted. Measurement of HDL uptake was carried out at 37°C by incubating liver slices with [³H]-CE-HDL under slight agitation for 3 h. Then, slices were washed and homogenized in PBS. Radioactivity recovered in the homogenate represented the amount of HDL uptaken by the liver cells.

*,†Statistically different at P < 0.05.