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. 2010 Jan 27;59(4):935–946. doi: 10.2337/db09-0404

FIG. 6.

FIG. 6.

OPN interferes with hepatic STAT3 activation in obesity. A–C: Obese high-fat diet–fed (HF) mice were treated with OPN-neutralizing (Anti-OPN) or control (n = 8 per group) antibody. STAT phosphorylation was determined by immunhistochemical analysis of pSTAT(Tyr705) on paraffin sections of livers isolated from high-fat diet–fed mice after anti-OPN or control treatment. A: Representative immunohistochemical pictures are given in 80- and 200-fold magnification, respectively. The representative TissueFaxs scattergramm shows the percentage of pSTAT(Tyr705) positive cells in liver; (n = 5 per group). B: Quantification of pSTAT3-positive cells determined by TissueFaxs. C: Immunoblot analysis of STAT3 tyrosine 705 phosphorylation in HepG2 cells. Cells were stimulated or not with 0.5 μg/ml human recombinant OPN for 30 min and 2 days followed by immunoblotting for pSTAT3(Tyr705) and STAT3. A representative blot is given along with mean ratios. ***P ≤ 0.001. (A high-quality digital representation of this figure is available in the online issue.)