TABLE 3.
Physiological parameters determined in mice 2 weeks after injection of empty pcDNA3.1 vector or pcDNA-2J3+
| Variables | C57BL/6 | C57BL/6 + pcDNA3.1 | C57BL/6 + p2J3+ | db/db | db/db + pcDNA3.1 | db/db + p2J3+ |
|---|---|---|---|---|---|---|
| Glucose (mmol/l) | 10.48 ± 0.57 | 9.78 ± 0.38 | 9.70 ± 0.60 | 21.99 ± 1.83* | 23.19 ± 2.51* | 23.15 ± 2.36 |
| Triglycerides (mmol/l) | 1.2 ± 0.15 | 1.5 ± 0.22 | 1.43 ± 0.14 | 2.26 ± 0.28* | 2.63 ± 0.00.32* | 2.57 ± 0.37 |
| Insulin (mIU/l) | 2.06 ± 0.35 | 2.13 ± 0.36 | 1.46 ± 0.31 | 7.66 ± 1.33* | 7.25 ± 0.87* | 8.25 ± 1.96 |
| Fasting glucose (mmol/l) | 5.54 ± 0.33 | 5.67 ± 0.26 | 5.18 ± 0.40 | 17.52 ± 3.20* | 18.80 ± 2.35* | 12.65 ± 1.14† |
| Fasting insulin (ng/ml) | 0.23 ± 0.02 | 0.22 ± 0.03 | 0.23 ± 0.01 | 2.49 ± 0.57* | 2.618 ± 0.19* | 1.43 ± 0.36† |
| HOMA-IR | 0.053 ± 0.006 | 0.055 ± 0.005 | 0.052 ± 0.002 | 2.19 ± 0.72* | 2.26 ± 0.45* | 0.81 ± 0.24† |
| Body weight (g) | 25.48 ± 0.61 | 25.45 ± 0.68 | 25.27 ± 0.47 | 45.16 ± 0.91 | 45.06 ± 0.97 | 45.78 ± 1.57 |
Serum glucose, serum insulin, insulin resistance (HOMA-IR), and serum triglyceride levels were significantly higher in the diabetic phenotype of db/db mice (injected with the empty pcDNA vector) compared with those in C57BL/6 mice. Injection of CYP2J3+ significantly reduced insulin resistance (HOMA-IR) in db/db mice, whereas injection of the empty pcDNA vector did not.
*P < 0.05 vs. C57BL/6 group;
†P < 0.05 vs. db/db + pcDNA3.1 group; n = 8 per group.