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. Author manuscript; available in PMC: 2010 Mar 24.
Published in final edited form as: Proteins. 2009 Feb 1;74(2):400–416. doi: 10.1002/prot.22143

Table 4.

Rotamer-dependent helical consensus sequence templates derived from the database and comparison to previous designed protein sequences.

A. Optimal sequences by rotamer (Contact residues highlighted in grey)
Ligand Histidine Rotamer Sequence
t73 IInline graphic XXXInline graphicXXHInline graphicXXInline graphicIInline graphic
m166 Inline graphicXXInline graphicXAInline graphicHXXInline graphic
t-86 AHXXInline graphicXXInline graphic
m80 Inline graphicKXInline graphicSXInline graphicHXX
B. The t73 sequence template compared to previous dihelical bis-histidine designs
Protein Sequence Reference
optimal t73 IInline graphicXXXInline graphicXXHInline graphicXXInline graphicIInline graphic this work
HP-1 EIWKQHEEALKKFEEALKQFEELKKL (22)
ME1 ILLASYGHRRLRKK (9)
D2-heme REKHRALAEQVYATGQMLKN (8)
H2α17-FF AEAFFKAHAEFFAKAA (26)
Δ7-His EIWKLHEEFIKLFEERIKKL (25)
6H7H DKWKQHHQEFKQFLKELKQKLEEIA (27)
AP1 DPLVVAASIIGILHFIEWILDRGGNGEIFKQ (18)
PAsc QQKALQTAKEFQQAMQKHKQY (34)
MOP-1 NALELHEKALKQLEELLKKL (35)
C. The m166 sequence template compared to the previous C-type cytochrome design
Protein Sequence Reference
optimal m166 Inline graphicXXInline graphicXAInline graphicHXXInline graphic this work
cHH ELLKLHEELLKKFEECLKLHEERLKKL (10)