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. Author manuscript; available in PMC: 2010 Mar 24.
Published in final edited form as: J Biol Chem. 2006 Nov 7;282(1):115–123. doi: 10.1074/jbc.M609830200

FIGURE 1.

FIGURE 1

A, construction of the transgene, pCaMKII-MAOcDNA (see text for details). Abbreviations of the restriction sites are as follows: E, EcoRI; S, SacI, B, BamHI; K, KpnI; N, NotI; and Ev, EcoRV. B, identification of forebrain transgenic mice by PCR. The 0.87-kb PCR product band was only found in forebrain transgenic mice (n = 7) and not in wild type (n = 2) or MAO A KO mice. Abbreviations used are as follows: WT, wild type mice; T, forebrain transgenic mice; KO, MAO A knock-out mice. C, forebrain-specific expression of hMAO A protein and RNA by Western blot and RT-PCR. F, frontal cortex; C, cerebellum; H, hippocampus; L, liver.