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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Pediatr Clin North Am. 2010 Feb;57(1):83–96. doi: 10.1016/j.pcl.2009.11.002

Figure 1. Generating CTLs by Repeated Ex-vivo stimulation.

Figure 1

Low frequency virus specific CTLs in a peripheral blood mononuclear cell population are expanded by primary and secondary stimulation with antigen expressed on antigen presenting cells followed by expansion with IL2. The resulting population is enriched for T cells specific for the viral antigen.