Fig. 1. SPR sensorgrams of binding of lipid-free apoE3 to immobilized HS.
The inset shows the fitting of experimental data (●) with the two-state binding model, where A + B ⇔ AB ⇔ ABx (see “Experimental Procedures”). Simulated curves displaying the initial binding (AB) and subsequent binding (ABx) are the additive components from the fitted curve (solid line). RU, resonance units.