Fig. 5. Effect of C-terminal truncation on the interaction of lipid-free apoE3 with HS.
A, SPR sensorgrams of binding of apoE3 C-terminal-truncated mutants to HS. apoE3 (a), apoE3 (1–272) (b), apoE3 (1–260) (c), and apoE3 22-kDa (d). The protein concentration was 30 μg/ml. B, Free energy for each binding steps of apoE3 C-terminal-truncated mutants.