Table 1.
VHRev | (GC)AGGC(GC)CAGCTGCAG(GC)AGTC(AT)GG |
VHFor | GGTCCCTTGGCCCC |
VLRev | GACATT(GC)(AT)G(AT)TGAC(GC)CAGTCTCC |
VLFor | ACGTTTGATCTCGAGCTTGGTCCC |
VHRevEcoRI | TTTGAATTCGAGGCGCAGCTGCAGGAGTC |
VHLinkFor | TCCGCCTGAACCGCCTCCACCTGAGGAGACGGTGACCGTGGTCCC |
VLLinkRev | GGAGGCGGTTCAGGCGGAGGTGGCTCTGGCGGTGGCGGATCGGACATTGTGATGACGCAGTCT |
VLForSalI | TTTGTCGACACGTTTGATCTCGAGCT |
Restriction sites EcoRI and SalI used for cloning the scFv are underlined