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. 2009 Dec 10;86(4):1067–1075. doi: 10.1007/s00253-009-2379-8

Table 1.

List of primers used for the generation of synthetic genes encoding scFv3G1

VHRev (GC)AGGC(GC)CAGCTGCAG(GC)AGTC(AT)GG
VHFor GGTCCCTTGGCCCC
VLRev GACATT(GC)(AT)G(AT)TGAC(GC)CAGTCTCC
VLFor ACGTTTGATCTCGAGCTTGGTCCC
VHRevEcoRI TTTGAATTCGAGGCGCAGCTGCAGGAGTC
VHLinkFor TCCGCCTGAACCGCCTCCACCTGAGGAGACGGTGACCGTGGTCCC
VLLinkRev GGAGGCGGTTCAGGCGGAGGTGGCTCTGGCGGTGGCGGATCGGACATTGTGATGACGCAGTCT
VLForSalI TTTGTCGACACGTTTGATCTCGAGCT

Restriction sites EcoRI and SalI used for cloning the scFv are underlined