Fig. 6.

Stimulation of the Nrf2 stress response pathway does not require the Chd6 exon 12. a Treatment of MEF cells with Nrf2 pathway stimulants CDDO or Sulforaphane increased the expression of Nqo1 in Exon 12 −/− MEF cells and WT MEFs to similar levels. qPCR analysis of the level of Nqo1 normalized to β-actin expression levels. b Increased expression of Nrf2 target genes results in protection from ROS challenge at similar levels in Exon 12 −/− MEF cells and WT MEF cells. MEFs pretreated with control or CDDO were labeled with DCFDA and challenged with tBHQ. The level of DCFDA fluorescence was measured by FACScan, and the change in mean fluorescence intensity was measured as treated over untreated levels. The average of six experiments is shown