Figure 1.

Effects of HDAC inhibition (by TSA treatment) on the expression of MCL1 mRNA. (A) The expression levels of MCL1 mRNA in an isogenic ATM^- cells and its ATM⁺ parental control cells treated with TSA for 24 h were determined using RT-PCR. GAPDH levels were used to confirm equal amounts of input RNA between samples. (B) The induction of MCL1 transcription by TSA in the TSA-treated (TSA) and non-treated (none) ATM^- and ATM⁺ cells was analyzed by quantitative real-time RT-PCR. All reactions were normalized to GAPDH. (C) The relative expression levels of survivin mRNA in the TSA-treated and non-treated cells were monitored by quantitative real-time RT-PCR. (D) Expression of MCL1 transcripts in the presence and absence of TSA was analyzed by quantitative real-time RT-PCR in HCT116 and U87MG cells. (E) The expression levels of MCL1 mRNA in HCT116 cells expressing wild type (ATM-WT) or kinase-dead mutant type (ATM-KD) ATM were determined using real-time PCR.