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. 2010 Feb 11;42(3):223–232. doi: 10.3858/emm.2010.42.3.024

Figure 2.

Figure 2

Effect of DDS on paraquat (PQ)-induced cytosolic or mitochondrial superoxide anion generation in HDFs. HDFs were seeded to 96 well plate for 24 h and pretreated with various concentration of DDS (0.1, 1, 5, and 20 µM), DPI (5 µM) for 30 min, and NAC (2 mM) for 3 h. The cells were then treated with 1 mM paraquat for 30 min. After paraquat treatment, the cells were incubated with the oxidant-sensitive fluorescent dyes (A) dihydroethidium (DHE) (5 µM, cytosol specific) and (B) MitoSOX Red (5 µM, mitochondria specific), respectively, for 15 min at 37℃. Changes in fluorescence were monitored with a multiwell plate reader using an excitation and emission wavelength of 515 and 590 nm (dihydroethidium), 520 and 580 nm (MitoSOX Red), respectively. Results are expressed as % of control rate. The data shown are representative of six experiments. #P < 0.005 relative to CC, *P < 0.005 relative to C.