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. 2010 Mar 3;4:3. doi: 10.3389/neuro.06.003.2010

Figure 1.

Figure 1

Confocal images of the individual T. castaneum brain stained with an antibody against synapsin which was used as template for the VIB protocol. (A, B) Single optical sections through the brain in the frontal (A) and horizontal plane (B). All manually labeled neuropil areas are shown as reconstructed in AMIRA. Labels of paired neuropils are only visualized in the left hemispheres, to provide a comparison to the unlabeled neuropils of the right hemispheres. (A) Frontal sections with the positions a–f from anterior to posterior and as described in (D). (B) Horizontal sections with the positions a´–c´ from dorsal to ventral as described in (D). (C) The color code of the labeled neuropils is consistent with Brandt et al. (2005), Kurylas et al. (2008), and el Jundi et al. (2009). AL, antennal lobe; Ca, Calyx; CBL, lower unit of the central body; CBU, upper unit of the central body; aMe, accessory medulla; Lo, lobula; LoP, lobula plate; Me, medulla; No, nodulus; PB, protocerebral bridge; and Pe, pedunculus with lobes. (D) Volume rendered (Voltex) view of the template brain from dorsal and frontal. The sections (a–f) and (a′–c′) represent the positions of the optical sections in (A,B). Orientation bars, p, posterior; m, median; d, dorsal. All scale bars, 50 μm.