Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Mar 22;188(6):953–969. doi: 10.1083/jcb.200908133

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 Cevik et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 7. — arl-13 interacts synthetically with ciliopathy/ciliary transport genes to maintain cilium structure/morphology and IFT. (A) Compared with single mutants, arl-13;klp-11, arl-13;bbs-8, and arl-13;nph-4 mutants are SynDyf, failing to take up dye. Merged DIC–fluorescence head images from single and double mutants after a DiI uptake assay (arrows denote dye uptake) are shown. (B and C) PHA/B cilia morphology defects are enhanced in double mutants of arl-13 and ciliopathy/ciliary transport genes. B shows cilium morphology data from worms expressing a PHA/B cilium marker (srb-6p::gfp). Data for kinks/bulges represent the percentage of cilia with these defects. n, number of animals assayed for cilium length; N, number of cilia assessed for ciliary kinks/bulges; N*, number of cilia assessed for bulges. C shows fluorescence images of PHA/B cilia in single and double mutants. Arrowheads indicate axonemal bulges, and asterisks indicate the cilia base. (D) Anterograde IFT particle formation (marked by KAP-1::GFP and DYF-2::GFP) is defective in arl-13;dyf-5 double mutants compared with single mutants. Fluorescence images, kymographs, and kymograph schematics of single and double mutants expressing kap-1::gfp or dyf-2::gfp are shown. (E–G) Quantitative analysis of anterograde IFT kymographs (from animals expressing dyf-2::gfp). Unlike single mutants, in many arl-13;dyf-5 double mutants, moving IFT assemblies are not found (E) or are severely reduced in number (F). Fewer moving IFT particles also found in arl-13;bbs-8 double mutants compared with single mutants (G). (F and G) Error bars indicate SEM. Bars: (A) 10 µm; (C) 3 µm; (D) 2 µm.