Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Mar 22;188(6):809–820. doi: 10.1083/jcb.201001006

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 Liu et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — PP1γ is recruited to kinetochores and dephosphorylates an Aurora B substrate as centromere tension is established. (A–C) HeLa cells transfected with a kinetochore-targeted Aurora B phosphorylation sensor, with or without KNL1 siRNA, were imaged live during recovery from nocodazole (30 ng/ml). Cells were followed for 33 min, which was sufficient time for control cells to reach metaphase. Cells rarely entered anaphase during this time, and any anaphase cells were excluded from the analysis. Representative images (A) show YFP emission. At each time point, the percentage of kinetochores aligned at the metaphase plate was determined (B) and the YFP/TFP emission ratio was calculated (C). Each data point represents seven cells, >15 kinetochores per cell. (D) Images of HeLa cells stably expressing GFP^LAP-PP1γ in early prometaphase, metaphase, or treated with nocodazole. (E) The relative intensity of GFP-PP1γ at kinetochores was calculated during recovery from nocodazole (30 ng/ml). n = 6 cells, multiple kinetochores per cell. Bars, 5 µm.