Figure 5.

Discontinuous adherens junctions are distinct from focal adhesions. Confluent human umbilical vein endothelial cells were stimulated with 10 ng/ml tumour necrosis factor-α as in Figure 4. Cells were fixed and stained with TRITC-phalloidin, in order to detect actin filaments, with antibodies to β-catenin, phosphorylated FAK (p(Y397)FAK) (A) or TRITC-phalloidin and antibodies to detect talin and β-catenin (B). Bottom panels in (A) show the red-boxed area from the top panels (merge) at higher magnification.