Table 2.
Strains and plasmids used in this study.
| Strain or Plasmid | Genotype and Description | Reference |
|---|---|---|
| Strains | ||
| B. burgdorferi | ||
| B31-A | High passage non-infectious wild type | [42] |
| RR04 | StrR; B31-A putative β-N-acetylhexosaminidase (bb0002) mutant | This study |
| RR53 | KanR; B31-A putative β-glucosidase (bb0620) mutant | This study |
| RR60 | StrR KanR; B31-A double mutant for bb0002 and bb0620 | This study |
| RR34 | StrR; B31-A chbC mutant | This study |
| JR14 | StrR KanR; RR34 complemented with BBB04/pCE320 | This study |
| A74 | CoumR; B31-A rpoS mutant | [42] |
| E. coli | ||
| DH5α | supE44 F- ΔlacU169 (ϕ80lacZ ΔM15) hsdR17 relA1 endA1gyrA96 thi-1relA1 | [43] |
| Plasmids | ||
| pKFSS1 | StrR; B. burgdorferi shuttle vector, cp9 based | [37] |
| pBSV2 | KanR; B. burgdorferi shuttle vector, cp9 based | [38] |
| pCE320 | KanR ZeoR; B. burgdorferi shuttle vector, cp32 based | [40] |
| pBB0002.7 | StrR; aadA::bb0002 | This study |
| pBB0620.5 | KanR; kan::bb0620 | This study |
| pBBB04.5 | StrR; aadA::bbb04 | This study |
| BBB04/pCE320 | KanR; bbb04 complementation construct | This study |