Table 3.

Oligonucleotide primers used in this study

Primer Name	Sequence (5'→3')
5'BB0002mutF (KpnI)	GCTAGGGTACCACATTGCCTTTATCGGAATATTGACATC
5'BB0002mutR (XbaI)	GCTAGTCTAGAAAGATGCGGAGCAGACAAAGGGAT
pKFSS1 R1	TGATGAACAGGGTCACGTCGTC
3' BB0002mutF (XmaI)	GCTAGCCCGGGCGATATTAAGCTCTTGAACATTCTTAAA
3'BB0002mutR (SacII)	GCTAGCCGCGGTAGTGCTATTAGTGCTTTATCTTTATTG
5'BB0620mutF3 (KpnI)	GCTAGGGTACCTACTTTGAATTTTGAATATGGAG
5'BB0620mutR2 (SalI)	GCTAGGTCGACTACCCAAATCAATCAATCAC
pBSV2 R1	TTATTATCGTGCACTCCTCCCGGT
3'BB0620mutF2 (SacII)	GCTAGCCGCGGCGTATCCCAAAAATCAATAGAAAA
3'BB0620mutR2 (AatII)	GCTAGGACGTCATGCAATCACCGCAATAGAAGCGG
5'BBB04mutF2 (BamHI)	GCTAGGGATCCGAATAAGTAGCTTTACGTCT
5'BBB04mutR2 (PstI)	GCTAGCTGCAGTACCAACAGTGGTATGTTGA
3'BBB04mutF1 (XmaI)	GCTAGCCCGGGCCAATTTTGCTAGCAATAGGA
3'BBB04mutR1 (SacII)	GCTAGCCGCGGGCATCTGGATTTAGGTCTGCTTTGA
BBB04 complement F1	GCTTCATTACTTCAACAGGACGACG
BBB04 complement R1	TCGCTAAGGCGTGTCTCAGCAATA
chbC F1	GGGAATTCAGCCCAATTCATGGTTTCC
chbC R1	GGCGGAACAGACTCTGGAAGCTTAAT
BB0002 CF1	ATGGACTTTTTAAAAACCTTTTCTTTTTTGTTTTTTAGC
BB0002 CR1	CTAAGGAATGAGTACTATATTGACACCCGA
BB0620 mut confirm F1	TCAAGAGTGGTATTGCCGTGTCCT
BB0620 mut confirm R1	ACTTGAACCCACGACAACTCGGAT
BBB04 mut confirm F1	AGCAGCATCTCCACCGTAAGGTAT
BBB04 mut confirm R1	CACCAGAGTAAGCTACAACAGGCA