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. 2010 Mar 6;3:6. doi: 10.1186/1757-2215-3-6

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Copyright ©2010 Jiang et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Validation of differentially expressed genes by real-time qPCR. Relative quantification of ten representative genes was performed. The method of Livak and Schmittgen (2001) was used to calculate the relative expression ratio (RER) that were normalized to a housekeeping gene 18S. Normal oocyte developmental competence (NDC) (solid bar) were expressed over poor oocyte developmental competence (PDC) (open bar), positive ratio refers to genes up-regulated, negative ratio indicated gene down-regulation, by which real-time qPCR data in the gene regulation trend (up- vs. down-regulation) were consistent with results obtained from microarray, of which the expression level of Lox (asterisk) was significantly higher in NDC in comparison to PDC (P < 0.05).