Table 1. Effect of the phytaspase overproduction or deficiency on TMV accumulation in tobacco Samsun NN plants.

Plant	Amount of TMV antigen (μg/g leaf)
	30°C → 24°C	30°C
wt	30±6	115±8
OE	6±2	110±7
KD	95±8	120±8
Accumulation of TMV in wild-type (wt) and transgenic N. tabacum Samsun NN plants overexpressing (OE; OE-1 line) or silencing (KD; KD1-1 line) tobacco phytaspase, was determined by ELISA using serial dilutions of TMV preparation as concentration standards. TMV-infected tobacco plants were incubated at 30°C for 24 h and then transferred to 24°C. The samples were taken 24 h after the temperature shift. Control plants were incubated at 30°C for 48 h. Data shown are means±s.d. of three independent experiments with three replicates each. Data were typical of all generated KD and OE transgenic lines, respectively.