Table 2.
Effect of eukaryotic translation initiation factor 5A (eIF5A) on serum cytokine concentrations 8 h after intraperitoneal challenge with lipopolysaccharide (LPS).
| Cytokine | Serum concentration, mean ± SE, pg/mL | P | |
|---|---|---|---|
| After receipt of eIF5A siRNAa |
After receipt of control siRNAa |
||
| IL-1α | 580.468 ± 67.804 | 859.7 ± 139.3 | .09 |
| IL-1β | 1003.4 ± 171.9 | 1994.4 ± 326.7 | .01 |
| IL-2 | 78.925 ± 11.5 | 115.429 ± 14.6 | .06 |
| IL-3 | 48.2 ± 7.8 | 66.7 ± 8.4 | .1 |
| IL-4 | 10.6 ± 1.5 | 12.8 ± 1.3 | .3 |
| IL-5 | 82.972 ± 18.6 | 87.8 ± 14.0 | .8 |
| IL-6 | 246,335.3 ± 119,250.6 | 674,670.4 ± 20,9941.0 | .08 |
| IL-10 | 241.0 ± 14.3 | 471.0 ± 84.6 | .03 |
| IL-12 (p40) | 57,345.7 ± 13,116.8 | 112,535.7 ± 20,938.3 | .04 |
| IL-12 (p70) | 1992.6 ± 342.7 | 3292.1 ± 687.4 | .1 |
| IL-17 | 1193.8 ± 168.6 | 1827.7 ± 403.1 | .2 |
| IFN-γ | 644.5 ± 225.9 | 940.3 ± 172.1 | .3 |
| GM-CSF | 447.3 ± 48.6 | 519.0 ± 56.4 | .3 |
| MIP-1α | 783.4 ± 118.7 | 1175.7 ± 162.9 | .07 |
| RANTES | 23,853.3 ± 7903.5 | 43,711.0 ± 12,594.7 | .2 |
| TNF-α | 1007.7 ± 135.9 | 1424.9 ± 191.7 | .09 |
NOTE. Mice were treated with eIF5A siRNA 48 and 24 h before challenge with 5 mg/kg LPS. The mice were euthanized 8 h after challenge with LPS for evaluation of serum concentrations of cytokines. GM-CSF, granulocyte-macrophage colony-stimulating factor; IFN, interferon; IL, interleukin; MCP, monocyte chemoattractant protein; MIP, macrophage inflammatory protein; RANTES, regulated on activation, normally T cell expressed and secreted; TNF, tumor necrosis factor.
a
There were 10 mice per experimental group.