Figure 4.

Chromosome mapping of wheat plastid ACCase genes. (a) Southern analysis of Chinese Spring NT lines. DNA was digested with HindIII. (b) Genetic maps of chromosome 2A and 2D, and consensus physical map of wheat chromosome 2. Position of Acc-1 genes was revealed by hybridization with cDNA probe ucg1. Names of markers, distances in centimorgans (genetic maps), and distances of the deletion breakpoints of various deletion lines from the centromere as fraction of the arm length (physical map) are shown. Lines connecting the maps indicate positions of markers in common, including Xucg1(ACCp) marked with the dashed line, and the position of the centromere marked on the genetic maps with a solid oval symbol. The chromosome 2A map, obtained from the GrainGenes database (http//:wheat.pw.usda.gov/graingenes.htm/.) was published previously (36).