Table 1.

Comparison of the amino acid sequence of wheat plastid ACCase deduced from cDNA sequence (pcw, accession number AF029895 with those deduced from gene sequence (Acc-1,1, Acc-1,2) and with sequences of some other biotin-dependent carboxylases

Species	Location	Identity, %
		Full length	Biotin carboxylase domain	Accession no.
Multidomain eukaryotic-type ACCases
Triticum aestivum Acc-1,1	Plastid	—	99.5	AF029897
Triticum aestivum Acc-1,2	Plastid	—	100	AF029896
Zea mays	Plastid	80	93	U19183
Brassica napus	Plastid	60	80	X77576
Brassica napus	Cytosolic	—	82	Y10301
Triticum aestivum	Cytosolic	67	81	U39321
Arabidopsis thaliana	Cytosolic	65	82	L27074
Medicago sativa	Cytosolic	66	84	L25042
Homo sapiens	Cytosolic	37	59	X68968
Saccharomyces cerevisiae	Cytosolic	38	57	M92156
Prokaryotic-type carboxylases
Nicotiana tabacum*	Plastid	—	33	L38260
Anabaena 7120*	Bacterial	—	35	L14862
Homo sapiens PCCase†	Mitochondrial	—	35	M22631
Arabidopsis thaliana MCCase‡	Mitochondrial	—	34	U12536

A dash indicates sequence not available or not found as one colinear contig in the prokaryotic-type carboxylases. Sequence alignments were created by using clustal W (22). 

^*Biotin carboxylase subunit of ACCase. 

^†Biotin carboxylase–biotin carboxyl carrier subunit (α) of propionyl-CoA carboxylase. 

^‡Biotin carboxylase-biotin carboxyl carrier subunit of methylcrotonyl-CoA carboxylase.