Fig. 4.

Confocal microscopy analysis of Cos1-AVR and Cos1-NAVR permanent cell transfectants. A: Cos1-AVR cells imaged in phase contrast (1); merged confocal image of Cos1-AVR cells with immunofluorescence of AVR in cytoplasm and cell membrane (red), in contrast to blue Hoechst stain for nuclei (2); red AVR+ fluorescence alone (3). B: Cos1-NAVR cells imaged in phase contrast (1); merged confocal image with NAVR immunofluorescence (red) detected in cytoplasm and cell membrane in contrast to blue nuclear stain (2); red NAVR+ fluorescence alone (3). C: Cos1-NAVR cells imaged in phase contrast (1); merged confocal image with NAVR immunofluorescence (red) detected in cytoplasm and nuclear membrane surrounding blue-stained nuclei (2); red NAVR+ fluorescence alone (3).