Figure 3.—

Abundance of RAD-51 foci on X chromosome(s). (A) Nucleus showing RAD-51 focus on X (arrow). Nucleus labeled with α-RAD-51 (red) and α-HIM-8 (green), counterstained with DAPI (blue). Scale bar, 5 μm. (B) Histograms representing quantification of RAD-51 foci on X chromosome(s) in N2 XX, tra-2 XX males, N2 X0, fem-3(e1996) X0, and him-8(me4) XX. Y-axis indicates percentage of nuclei that contain 0, 1, or 2–3 RAD-51 foci per X chromosome for early pachytene (white), midpachytene (gray), and late pachytene (black).