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. 2010 Mar;184(3):613–628. doi: 10.1534/genetics.109.110338

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Figure 6.— — Chromatin/transcriptional state of X chromosome(s). Immunolocalization of (A) H3dimethylK9; scale bar, 50 μm and (B) H3methylK4 (red) counterstained with DAPI (blue); scale bar, 5 μm in N2 XX, N2 X0, fem-3(e1996) X0, and him-8(me4) XX germ lines. Images were captured with same exposure time. Arrowheads indicate X chromosome(s) as determined by HIM-8 staining (green); HIM-8 is not present on diakinesis or spermatocyte nuclei. (C) In situ hybridization of oocyte-enriched X-linked F52D2.2 in N2 XX, N2 X0, fem-3(e1996) X0, and him-8(me4) XX germ lines. fem-3(e1996) X0 worms were mated with N2 males. Asterisk denotes proximal gonad. (D) Immunolocalization of H3dimethylK9 (green) counterstained with DAPI (blue); scale bar, 5 μm in zim-2(tm574) XX and fem-3(e1996); zim-2(RNAi) X0 germ lines. Arrowheads indicate X chromosome(s) (HIM-8; red); arrows indicate chromosomes with H3dimethlyK9 that presumably represent chromosome V. (E) H3methylK4 (red) counterstained with DAPI (blue); scale bar, 5 μm in zim-2(tm574) XX and fem-3(e1996); zim-2(RNAi) X0 germ lines. Arrowheads indicate X chromosome(s) (HIM-8; green). Late pachytene (LP), diplotene (DP), diakinesis (DI), and spermatocyte (Sc).

Figure 6.— — Chromatin/transcriptional state of X chromosome(s). Immunolocalization of (A) H3dimethylK9; scale bar, 50 μm and (B) H3methylK4 (red) counterstained with DAPI (blue); scale bar, 5 μm in N2 XX, N2 X0, fem-3(e1996) X0, and him-8(me4) XX germ lines. Images were captured with same exposure time. Arrowheads indicate X chromosome(s) as determined by HIM-8 staining (green); HIM-8 is not present on diakinesis or spermatocyte nuclei. (C) In situ hybridization of oocyte-enriched X-linked F52D2.2 in N2 XX, N2 X0, fem-3(e1996) X0, and him-8(me4) XX germ lines. fem-3(e1996) X0 worms were mated with N2 males. Asterisk denotes proximal gonad. (D) Immunolocalization of H3dimethylK9 (green) counterstained with DAPI (blue); scale bar, 5 μm in zim-2(tm574) XX and fem-3(e1996); zim-2(RNAi) X0 germ lines. Arrowheads indicate X chromosome(s) (HIM-8; red); arrows indicate chromosomes with H3dimethlyK9 that presumably represent chromosome V. (E) H3methylK4 (red) counterstained with DAPI (blue); scale bar, 5 μm in zim-2(tm574) XX and fem-3(e1996); zim-2(RNAi) X0 germ lines. Arrowheads indicate X chromosome(s) (HIM-8; green). Late pachytene (LP), diplotene (DP), diakinesis (DI), and spermatocyte (Sc).