Figure 2.—

Activation of fl by blue light and the white collar complex. (A) The fl gene is activated by blue light. Total RNA was isolated from vegetative mycelia of the wild type exposed to white light (16.5 W/m²), blue light (2.6 W/m²), or red light (1.8 W/m²) for 10 or 30 min or kept in the dark (D) prior to RNA isolation. (B) The activation of fl requires the WCC. Total RNA was isolated from vegetative mycelia of the wild type and the wc mutants that had been incubated in the dark for 48 hr (22°C) and exposed to white light during 10, 30, or 120 min or kept in the dark (D) prior to RNA isolation. The amount of fl and tub-2 RNAs were determined by quantitative RT–PCR. Each fluorescent signal was first normalized to the corresponding tub-2 signal to correct for loading errors and then was normalized to the RNA sample from wild-type mycelia kept in the dark. The plot shows the average and standard error of the mean of the relative photoactivation in two to nine experiments (A) or in two experiments (B) except one experiment for the wc strains after 10 or 120 min of light. Each RNA sample was quantified in one PCR experiment.