Figure 6.—

Additional SAGA subunits have postrecruitment functions. (A) The wild-type strain and strains deleted for individual genes encoding representative subunits of the SAGA complex were assayed using the tethering system. Serial dilutions were spotted on media with and without AT and incubated for 3 days. (B) CYC1 expression levels in the indicated strains during growth in glucose (uninduced: open bars) and ethanol (induced: solid bars) were measured by S1 nuclease protection. Mean ± SD of three separate biological replicates (independent cultures) is shown.