Figure 1.—

Model of Snf7 role in Rim101 processing and in ESCRT complex functions. On the left, ESCRT-I and -II recruitment of Vps20–Snf7 to the endosomal membrane leads to Snf7 interaction with the protease Rim13 and scaffold protein Rim20. Rim20 interacts with the C-terminal PEST-like domain of Rim101, and these interactions lead to Rim101 processing to its active form. On the right, ESCRT-I and -II recruitment of Vps20–Snf7 leads to downstream recruitment of Vps2/Vps24 and Bro1. Vps4 interacts with Snf7 to facilitate ESCRT-III dissociation from the membrane, and these interactions lead to multivesicular body formation.