Figure 2.—

FM 4-64 localization in snf7 mutants, including WT (DAY185), snf7Δ/Δ (DAY763), snf7Δ/Δ + SNF7-V5 (DAY980), rim20Δ/Δ (DAY1153), bro1Δ/Δ (DAY1156), vps4Δ/Δ (DAY1155), snf7Δ/Δ + snf7-2 (DAY982), snf7Δ/Δ + snf7-6 (DAY986), snf7Δ/Δ + snf7-15 (DAY994), snf7Δ/Δ + snf7-20 (DAY 999), snf7Δ/Δ + snf7-48 (DAY1027), and snf7Δ/Δ + snf7-49 (DAY1028). Strains were grown at 30° to mid-log phase in M199 (pH 8) medium and exposed to FM 4-64 for 15 min. Cells were washed and incubated in fresh M199 pH 8 medium for 90 min at 30°. Cells were placed on ice and NaF and NaN₃ were added prior to photographing. Arrows indicate Class E-like accumulations in snf7Δ/Δ strain.