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(A) Rim101 processing is affected by some snf7 mutants. Strains were grown to mid-log phase in M199 pH 8 medium before protein preparation. Equivalent protein amounts were analyzed by Western blotting analysis. FL, full length Rim101 (85 kDa); P1, processed form 1 of Rim101, the active form (74 kDa); P2, processed form 2 of Rim101, with unknown function (65 kDa). (B) Rim101 processing is decreased in the presence of only one SNF7 allele regardless of V5 epitope.
