Figure 9.—

C. albicans-mediated FaDu cell damage is affected by Rim101-dependent defects. (A) Both the rim101Δ/Δ mutant and snf7Δ/Δ mutant have severe FaDu cell damage defects, and the SNF7-V5 allele rescues most of the snf7Δ/Δ defect. FaDu monolayers were Cr⁵¹ labeled overnight, then washed and incubated with 1 × 10⁵ cells/ml C. albicans for 10 hr. Strains were tested in triplicate during each assay and compared with media-alone wells to measure specific Cr⁵¹ release. The assay was repeated at least three times; the figure denotes one representative assay. (B) Only mutant snf7 alleles with Rim101-dependent defects have damage defects. Assays were run as described in A, and mutant snf7 allele damage was compared to SNF7-V5 damage. Each strain was tested in triplicate during each assay, and assays were repeated twice for each mutant snf7 allele.