Figure 2.—

pSSZ1 plasmid restores ERAD-M in cdc48-10 but Ssz1p is not required for ERAD-M. (A) Turnover of 6myc-Hmg2 was measured in cdc48-10 (KFY194; open triangles) and cdc48-10 expressing pSSZ1 (closed circles). The degradation of 6myc-Hmg2 was followed at 37° as described in Figure 1A and plotted as described in Figure 1B. Values shown are means ± SEM of at least four independent experiments. (B) Turnover of 6myc-Hmg2 was measured in wild-type BY4741 (closed circles) and Δssz1 deletion strain derived from it (open circles). The degradation of 6myc-Hmg2 was followed at 30° as described in Figure 1A and plotted as described in Figure 1B. Values shown are means ± SEM of at least four independent experiments.