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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: J Mol Cell Cardiol. 2009 Mar 2;46(6):876–882. doi: 10.1016/j.yjmcc.2009.02.020

Figure 5.

Figure 5

Electrophoretic-mobility shift assays (EMSA): EMSA was performed using LightShift® Chemiluminescent EMSA Kit (Pierce) to assess the direct binding of the Biotin-labeled oligonucleotides corresponding to the putative PPRE sequence (−837 to −817) in the mouse Mfn2 promoter to endogenous PPARδ in nuclear extracts. Nuclear extracts with or without PPARδ were obtained from the α-MyHC-Cre or the CR-PPARδ−/− hearts, respectively. Experiments were performed three times with similar results.