Effects of RALT on cardiac hypertrophy in vivo. A, Statistical results of heart rate to body weight ratio, lung weight to body weight ratio, and the size of myocyte cross-sectional areas (n=200 cells per group) after 4-week infusion of Ang II or ISO (n=8). Heart and lung tissues were freshly isolated from each group, and heart weight/body weight and lung weight/body weight ratios were determined. C and D, Analysis of hypertrophic markers. Total RNA was isolated from hearts of mice of the indicated groups, and expression of transcripts for ANP, BNP, Myh-7, and Myh-6 induced by Ang II or ISO infusion was determined by real-time PCR analysis. Data represent typical results of 3 to 4 different experiments as mean±SE (n=5 mice per group). *P<0.01 vs WT/saline; §P<0.01 vs WT after Ang II or ISO infusion.