Figure 2.

Detection of COs in Both the Wild Type and zep1-1.

(A) zep1-1 pachytene chromosomes stained with 4',6-diamidino-2-phenylindole (DAPI).

(B) Magnified view of the boxed region in (A); visible COs of one chromosome arm are indicated by arrows.

(C) Pachytene chromosome in the wild type probed with OSJNBa0088I16 (red) and 5S rDNA (green) located near the end and close to the centromere, respectively, of the short arm of chromosome 11.

(D) Selected early diplotene chromosomes in the wild type probed with the same markers as in (C); arrows indicate COs.

(E) zep1-1 pachytene chromosomes probed with the same two markers.

(F) Magnified view of the boxed region in (E); arrows indicate COs.

Bars = 5 μm.